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Fırat Tıp Dergisi |
2006, Cilt 11, Sayı 2, Sayfa(lar) 088-092 |
[ Turkish ] [ Tam Metin ] [ PDF ] |
Measurement of Total Malondialdehyde in Plasma and Tissues by High-Performance Liquid Chromatography and Thiobarbituric Acid Assay |
Nurten TÜKÖZKAN, Hüsamettin ERDAMAR, Ilgım SEVEN |
Gazi Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı, ANKARA |
Objective: Malondialdehyde, a product of lipid peroxidation, is a good marker for development of oxidative stress.
Mateials and Methods: In this study MDA was determined in the tissues and plasma by means of thiobarbituric acid test and directly by reverse phase high-pressure liquid chromatography method after derivatisation with 2,4-dinitrophenylhidrazine. Results: We have found a significant difference between two methods by Mann-Whitney U test (p<0.001). There was the formation of non-lipidrelated, malondialdehyde like, TBA-reactive substance that leads to overestimation of the extent of lipid peroxidation. On the contrary, by direct HPLC method, there was a decrease of MDA from plasma and tissue serving as control. Conclusion: Our results indicated that TBA test is not a specific and reliable index of lipid peroxidation. ©2006, Fırat Üniversitesi, Tıp Fakültesi |
[ Turkish ] [ Tam Metin ] [ PDF ] |
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