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Fırat Tıp Dergisi
2008, Cilt 13, Sayı 3, Sayfa(lar) 167-170
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The Protective Effect of Nicardipine on Iron-Induced Purkinje Cell Loss in Rat Cerebellum: A Stereological Study
Ramazan KOZAN1, M. Ömer BOSTANCI2, Fatih SEFİL2, Faruk BAĞIRICI2
1Mustafa Kemal University, Faculty of Medicine, Department of Physiology, HATAY
2Ondokuz Mayis University, Faculty of Medicine, Department of Physiology, SAMSUN

Objective: The aim of the present study is to investigate the effect of nicardipine, a calcium channel blocker, on the neurotoxicity induced by intracerebroventricular (i.c.v.) iron injection in rats.

Materials and Methods: Animals were divided into three groups; control, iron and iron+nicardipine groups. Rats in iron and iron+nicardipine groups received i.c.v. FeCl3, while rats in control group received the same volume of saline. All animals were kept alive for ten days following the operation and animals in iron+nicardipine group were injected intraperitoneally nicardipine (10 mg/kg/day) once a day during this period. After ten days, all rats were perfused intracardially and cerebellar tissues were stained with Cresyl violet. Means of total Purkinje cells numbers in the cerebellum were estimated using the optical fractionator counting method.

Results: Means of total Purkinje cells numbers in the cerebellum as follows: 317182±9667, 209002±7836 and 265659±8291 in the control, iron and iron+nicardipine groups, respectively. Total number of Purkinje cells in iron and iron+nicardipine groups were significantly lower than control animals (p< 0.05). However, comparison between iron and iron+nicardipine groups revealed that nicardipine significantly attenuates the iron-induced Purkinje cell loss (p<0.05).

Conclusion: It has been shown firstly in the present study that an excessive amount of iron has a toxic effect on cereballar Purkinje cell in rats and this deleterious effect is protected by nicardipine, a calcium channel blocker. ©2008, Firat University, Medical Faculty


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