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Fırat Tıp Dergisi
2007, Cilt 12, Sayı 1, Sayfa(lar) 005-008
[ Turkish ] [ Tam Metin ] [ PDF ]
Analysis of Chromosome 8 Copy Number Changes in Colorectal Cancers by Fluorescence In Situ Hybridization (FISH).
Hüseyin YÜCE1, Ebru ETEM1, Şükriye Derya DEVECİ1, Osman DOĞRU2, İbrahim Hanifi ÖZERCAN3, Halit ELYAS1
1Fırat Üniversitesi Tıp Fakültesi Tıbbi Biyoloji ve Genetik Anabilim Dalı, ELAZIĞ
2Fırat Üniversitesi Tıp Fakültesi Genel Cerrahi Anabilim Dalı, ELAZIĞ
3Fırat Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı, ELAZIĞ

Objective: Colorectal cancer (CRC) is one of the most common malignancies worldwide. Colorectal carcinogenesis has associated with the progressive acquisition of a variety of genomic alterations by neoplastic cells, some of these have been linked to early stages of CRC development. The aims of this study (1) to identify alterations of chromosome 8 in primary colorectal carcinomas from Turkish patients and (2) to determine which alterations of chromosome 8 are early events during the development of colorectal carcinoma using fluorescence in situ hybridization (FISH).

Materials and Methods: To reveal the significance of genetic abnormalities of the chromosome 8, 28 colorectal tumors were analyzed using FISH. The centromeric-probe for chromosome 8 was used for FISH. In each case, at least 200 nuclei were scored for each hybridization.

Results: Monosomy in 3.6%, disomy in 39.3%, trisomy in 53.6% and tetrasomy in 3.6% of the analyzed adenomas were determined. Chromosome 8 gain was found in 5 of 8 (62.5%) nonpolypoid and 3of 9 (33.3%) polypoid cancers. There was statistically significant correlation between chromosome 8 gain and stage of CRC.

Conclusions: There are several reports of chromosome 8 gain in solid tumors. FISH is a useful method to detect genetic abnormalities in solid tumors. It was shown that chromosome 8 gain FISH associated with the stage of CRC. Chromosome 8 monosomy may be a early event in CRC. Further studies involving more patients need to determine the importance of this alteration in CRC. ©2007, Firat University, Medical Faculty

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